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KMID : 0604520190450020161
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Journal of the Society of Cosmetic Scientists of Korea
2019 Volume.45 No. 2 p.161 ~ p.173
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Studies on Antioxidant Activity and In Vitro Inhibitory Activity of Tyrosinase and Collagenase in Artocarpus nitidus subsp. lingnaensis (Merr.) F.M. Jarrett using 4 Parameter Logistic
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Son Kwang-Hee
Kim Young-Kook
Choi Sang-Ho
Zhang Zhiyun
Shin Dong-Ha
Lee Jong-Suk
Park Ho-Yong
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Abstract
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In this study, the antioxidative and inhibitory activity of tyrosinase and collagenase for the solvent extract and silica column fractions of Artocarpus nitidus were evaluated. The activities were quantified using the 4 parameter logistic. LC/MS analysis showed that the major component of the fractions was polyphenol and the total polyphenol content of the extract was 48.1 ± 2.6 mg GAE/g. The radical scavenging activities (SC50) for 1,1-diphenyl-2-picrylhydrazyl of the extract, fraction-1 and fraction-2 were 16.7, 42.0 and 10.1 μg/mL, respectively. The value for fraction-2 was the closest to ascorbic acid (1.5 μg/mL). The tyrosinase inhibitory activity of the extracts and the fractions showed IC50 of 64.9, 0.9 and 1.2 μg/mL, respectively, and overall activity was higher than that of kojic acid (7.4 μg/mL) and arbutin (119.0 μg/mL). In the experiment by zebrafish embryo, the whitening activity of fraction-2 (27.5%) was higher than that of kojic acid (18.6%), and there was no adverse effect up to 500 μg/mL of fraction-2. For the collagenase inhibitory activity, the samples showed IC50 of 139.8, 20.6, and 16.8 μg/mL, respectively, which were competitive to 1, 10-Phenanthroline (55.4 μg/mL). The extract and fraction-2 showed IC50 of 61.8 and 67.1 μg/mL for elastase. These results suggest that A. nitidus extract can be used as a cosmetic material useful for antioxidant, whitening, and prevention of skin aging without adverse effects.
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KEYWORD
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antioxidant, polyphenol, collagenase inhibition, tyrosinase inhibition, Artocarpus nitidus
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